InlB protein secreted by Listeria monocytogenes controls the pathogen interaction with macrophages

The virulence of gram-positive bacterium Listeria monocytogenes depends on its capacity to infect non-professional phagocytes and proliferate inside them. Listerias captured by mononuclear phagocytic cells during the infectious process are resistant lysosomal digestion can macrophages. Internalin B (InlB), one key pathogenicity factors L. monocytogenes, interacts with mammalian receptors c-Met gC1q-R. For epithelial cells, such interactions surface promote activation these cytoskeletal remodeling, which leads massive bacterial invasion into nonprofessional phagocytes. macrophages, contrast, nothing is known about role InlB in their apart from fact that both abundantly expressed macrophages participate development immune reactions. This study aimed at determination potential between We found 1) expression promoted a significant 3.5-fold increase rates capture macrophages; 2) 24 h fold number constituted 182.5 ± 16.7, 96 12 13.3 3 for EGDe∆inlB, EGDe EGDe∆inlB::pInlB strains, respectively; 3) strain, complemented plasmid copy inlB, produced 3.3fold higher than type strain EGDe. conclude negatively affects survival listeria results enable advanced understanding host-pathogen monocytogenes.